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为观察氯化汞致肝损伤及番茄红素的防护作用,为汞中毒的防治提供理论依据,将50只Wistar大鼠随机均分成对照、低汞、中汞、高汞、番茄红素干预组。前4组给予大豆油灌胃,第5组给予40 mg/kg番茄红素灌胃。2 h后,对照、低汞、中汞组分别皮下注射0.9%NaCl、2.2μmol/kg HgCl2和4.4μmol/kg HgCl2,高汞、番茄红素干预组皮下注射8.8μmol/kgHgCl2,注射容量均为5 ml/kg。每天染汞1次,染毒2 d。测定肝Hg含量、LDH和ALT活力。并进行肝组织病理学观察。结果显示,与对照组比较,随着染汞剂量的增加,肝汞、LDH和ALT水平均逐渐增加(P<0.05);肝组织出现病理形态损伤,组织变性坏死;肝组织出现异染色体边集、线粒体肿胀等超微结构损伤。与高汞组比较,番茄红素干预组大鼠肝汞含量未见明显改变;血清LDH和ALT活力分别降低至67.09%和29.01%;肝脏组织病理及超微结构损伤有所缓解。提示汞能蓄积于大鼠肝脏并对其造成损伤,番茄红素提前干预对汞致大鼠的肝损伤具有一定程度的拮抗作用。
In order to observe the protective effect of liver caused by mercuric chloride and the protective effect of lycopene, 50 Wistar rats were randomly divided into control, low mercury, middle mercury, high mercury and lycopene intervention groups . The first four groups were given intragastric administration of soybean oil, and the fifth group was given intragastric administration of 40 mg / kg lycopene. After 2 hours, 0.9% NaCl, 2.2μmol / kg HgCl2 and 4.4μmol / kg HgCl2 were injected subcutaneously into the control, low mercury and middle Mercury groups, respectively. High mercury and lycopene intervention groups were subcutaneously injected with 8.8μmol / 5 ml / kg. Mercury 1 day, exposure 2 d. Liver Hg content, LDH and ALT activity were measured. Liver histopathology was observed. The results showed that compared with the control group, the levels of mercury, LDH and ALT increased gradually with the increase of the dose of mercury (P <0.05). The pathological morphological changes of the liver tissue were observed and the degeneration and necrosis of the liver tissue occurred. , Mitochondrial swelling and other ultrastructural damage. Compared with the high mercury group, the hepatic mercury level did not change significantly in the lycopene intervention group; the activities of serum LDH and ALT decreased to 67.09% and 29.01% respectively; the histopathological and ultrastructural damage of the liver were relieved. It is suggested that mercury can accumulate in rat liver and damage it. Early intervention of lycopene can antagonize liver damage induced by mercury in rats to a certain extent.