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目的:探讨我国上海地区汉族人群的对氧磷酶1(PON1)基因T-107C、PON2 Ser311Cys基因多态性与脑梗死发病乃至颈动脉硬化之间的关系。方法:采用聚合酶链反应-限制性片段长度多态性技术,检测134例脑梗死患者和103名健康对照者PON1 T-107C、PON2 Ser311Cys的多态性基因型,并研究其对脑梗死发病的影响。结果:多因素多元逐步Logistic回归分析显示,三酰甘油、总胆固醇是脑梗死的独立危险因素;同时,脑梗死组的PON1 T-107C、PON2 Ser311Cys基因型及等位基因频率分布与健康对照组间差异均有统计学意义(P<0.05),且脑梗死合并颈动脉斑块患者与健康对照组间的PON1 T-107C、PON2 Ser311Cys基因型及等位基因频率分布差异也均有统计学意义(P<0.05)。结论:我国上海地区汉族人群PON1 T-107C、PON2 Ser311Cys这2个位点的基因多态性可能与脑梗死发病乃至颈动脉硬化有关。
Objective: To investigate the relationship between polymorphisms of PON1 gene T-107C and PON2 Ser311Cys and the incidence of cerebral infarction and even carotid atherosclerosis in Han population of Shanghai, China. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect polymorphisms of PON1 T-107C and PON2 Ser311Cys in 134 patients with cerebral infarction and 103 healthy controls. Impact. Results: Multivariate multiple stepwise logistic regression analysis showed that triglyceride and total cholesterol were independent risk factors for cerebral infarction. Meanwhile, the frequencies of PON1 T-107C and PON2 Ser311Cys genotypes and alleles in cerebral infarction group were significantly higher than those in healthy controls (P <0.05). There were also significant differences in the distribution of PON1 T-107C, PON2 Ser311Cys genotypes and allele frequency between cerebral infarction patients with carotid plaque and healthy controls (P <0.05). Conclusion: The genetic polymorphisms of PON1 T-107C and PON2 Ser311Cys in Shanghai Han population of China may be related to the onset of cerebral infarction and even carotid atherosclerosis.