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目的研究CDK2、P57在子宫内膜组织中的分布和表达,探讨其在子宫内膜腺癌发病中的作用。方法运用原位杂交方法检测CDK2、P57在14例正常子宫内膜、19例非典型增生子宫内膜、45例子宫内膜腺癌中的表达情况。结果 CDK2蛋白在正常子宫内膜、非典型增生子宫内膜、子宫内膜腺癌的阳性表达率分别为7.14%、36.84%、71.11%,各组之间的差异有显著性(P<0.05)。CDK2蛋白的阳性表达率在不同组织学分级、淋巴有无转移之间具有显著性差异(P<0.05),而在肌层浸润、不同手术一病理分期、年龄之间的差异均无显著性差异(P>0.05)。P57蛋白在正常子宫内膜、非典型增生子宫内膜、子宫内膜腺癌的阳性表达率分别为71.43%、52.63%和44.44%,各组之间的差异有显著性(P<0.05)。P57阳性表达缺失与组织学分级、淋巴有无转移有显著性差异(P<0.05),与子宫肌层浸润程度、不同手术-病理分期、年龄之间的差异无显著性差异(P>0.05);P57蛋白表达与CDK2呈负相关(P<0.05)。结论子宫内膜腺癌中存在CDK2蛋白的异常表达及P57蛋白表达下降或缺失,促进了细胞的生长和肿瘤的发展,是子宫内膜腺癌的发生、发展中的重要事件。
Objective To study the distribution and expression of CDK2 and P57 in endometrial tissue and explore its role in the pathogenesis of endometrial adenocarcinoma. Methods The in situ hybridization was used to detect the expression of CDK2 and P57 in 14 cases of normal endometrium, 19 cases of atypical hyperplasia and 45 cases of endometrial adenocarcinoma. Results The positive expression rate of CDK2 protein in normal endometrium, atypical hyperplasia endometrium and endometrial adenocarcinoma was 7.14%, 36.84%, 71.11%, respectively. There was a significant difference between the groups (P <0.05) . The positive expression rate of CDK2 protein in different histological grade, lymph node metastasis was significantly different (P <0.05), while in the muscle invasion, different surgical pathological stage, age, no significant difference (P> 0.05). The positive expression rates of P57 protein in normal endometrium, atypical hyperplasia endometrium and endometrial adenocarcinoma were 71.43%, 52.63% and 44.44%, respectively. There was significant difference between each group (P <0.05). The positive expression of P57 was not associated with histological grade and lymphatic metastasis (P <0.05), but there was no significant difference between the expression of P57 and the degree of myometrial invasion, different surgical-pathological stages and age (P> 0.05) ; P57 protein expression was negatively correlated with CDK2 (P <0.05). Conclusion The abnormal expression of CDK2 protein and the decrease or deletion of P57 protein in endometrial adenocarcinoma promote the cell growth and tumor development and are important events in the development of endometrial adenocarcinoma.