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哮喘是呼吸系统常见病之一，其发病机制至今未明。有研究表明，哮喘与原癌基因调控有关［１］。我们通过本实验探讨原癌基因在哮喘发病中的作用，拟从基因水平探索哮喘的发生机制。一、材料与方法盐酸胍、ＳＤＳ购自美国Ｓｉｇｍａ公司，［α－３２Ｐ］ｄＡＴＰ（放射比活度为
Asthma is one of the common respiratory diseases, the pathogenesis has not yet been identified. Some studies have shown that asthma and proto-oncogene regulation [1]. We explore the role of proto-oncogene in the pathogenesis of asthma through this experiment, and we want to explore the mechanism of asthma from the gene level. First, the material and methods Guanidine hydrochloride, SDS purchased from the United States Sigma, [α-32P] dATP (radioactivity ratio