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目的 观察日本血吸虫感染小鼠肝肉芽肿病变与一氧化氮 (NO)、Th1/Th2细胞因子水平的动态变化 ,探讨NO介导Th1/Th2免疫偏移在血吸虫卵肉芽肿病变中的作用。方法 采用石蜡切片 ,HE染色后观察感染小鼠肉芽肿病变。用硝酸还原酶法和ELISA夹心法分别检测日本血吸虫感染小鼠 0～ 12周血清及脾CD+ 4 T淋巴细胞培养上清NO、IFNγ和IL - 4表达水平 ,并进行相关分析。结果 感染小鼠 0～ 12周血清和脾CD+ 4 T淋巴细胞培养上清NO表达水平与小鼠肝肉芽肿病变动态相一致 ,并呈显著正相关 ,与IFNγ表达水平呈显著正相关 ,而与IL - 4在小鼠感染慢性期 (12周 )呈显著负相关。结论 NO在日本血吸虫感染小鼠肝肉芽肿病变过程中可能是一种与Th1/Th2细胞因子具有同样重要作用的调节因子 ,并可能是通过调节Th1/Th2细胞因子而发挥作用的 ,通过调控NO合成介导Th1/Th2免疫偏移可能是控制血吸虫卵肉芽肿病变的新途径
Objective To observe the dynamic changes of liver granuloma lesions and the levels of nitric oxide (NO) and Th1 / Th2 cytokines in mice infected with Schistosoma japonicum and explore the role of NO-mediated Th1 / Th2 immunostaining in the pathogenesis of Schistosoma granulosus. Methods Paraffin sections were used to observe the granuloma lesions in mice infected with HE. The levels of NO, IFNγ and IL - 4 in the serum of 0, 12 weeks and the supernatant of spleen CD + 4 T lymphocytes were detected by nitrate reductase and ELISA sandwich ELISA. Results The expression level of NO in serum and spleen CD + 4 T lymphocytes of infected mice from 0 to 12 weeks was consistent with that of mice with hepatic granuloma and was positively correlated with the expression of IFNγ, IL - 4 was significantly negatively correlated with chronic phase (12 weeks) in mice. Conclusion NO may play an important role in the pathogenesis of hepatic granuloma in mice infected with Schistosoma japonicum, and may play an important role by regulating Th1 / Th2 cytokines. By regulating NO Synthesis-mediated Th1 / Th2 Immunostaining May Be a New Way to Control the Egg Granuloma Lesions in Schistosoma