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目的探讨高压氧联合rh-EPO对窒息新生大鼠心肌细胞Bcl-2/Bax表达的影响,为临床治疗新生儿窒息后心肌损害提供分子理论依据。方法选取新生3~7 d的Wistar大鼠40只为研究对象,随机分为对照组、窒息组、rh-EPO组、高压氧组、高压氧联合rh-EPO组各8只,分别给予相应的干预措施。病理学检查及半定量分析常规HE染色,光镜下观察心肌病理改变。对各组心肌细胞采用免疫组织化学染色法检测Bcl-2/Bax蛋白表达。结果新生大鼠心肌细胞病理学观察显示:高压氧联合rh-EPO组心肌细胞结构基本正常,细胞界限欠清晰,心肌纤维排列基本正常;Bcl-2/Bax蛋白表达阳性细胞胞浆呈棕黄色染色。窒息组阳性细胞最多,高压氧联合rh-EPO组阳性细胞较少。结论新生大鼠窒息后采用高压氧与rh-EPO治疗,两者产生协同作用,通过增加Bcl-2表达、减少Bax表达而发挥心肌保护作用,Bcl-2/Bax比值明显增加。
Objective To investigate the effect of hyperbaric oxygen combined with rh-EPO on the expression of Bcl-2 / Bax in neonatal rat cardiomyocytes induced by asphyxia, and to provide a molecular basis for the clinical treatment of neonatal asphyxia myocardial damage. Methods 40 Wister rats aged 3-7 days were selected and randomly divided into control group, asphyxia group, rh-EPO group, hyperbaric oxygen group, hyperbaric oxygen group and rh-EPO group, Interventions. Pathological examination and semi-quantitative analysis of conventional HE staining, pathological changes observed under light microscope. The expression of Bcl-2 / Bax protein was detected by immunohistochemistry in each group of cardiomyocytes. Results The cardiomyocytes in neonatal rats showed that the cardiomyocytes in the hyperbaric oxygen combined with rh-EPO group were basically normal in structure, the cell boundaries were not clear, and the myocardial fibers were basically normal. The cytoplasm of Bcl-2 / Bax positive cells showed brownish yellow staining . Asphyxia group had the highest number of positive cells, while hyperbaric oxygen combined with rh-EPO group had fewer positive cells. Conclusions The neonatal rats are treated with hyperbaric oxygen and rh-EPO after asphyxia, and the synergistic effect of them can be obtained by increasing the expression of Bcl-2 and decreasing the expression of Bax, and the ratio of Bcl-2 / Bax is obviously increased.