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目的探讨将钙视网膜蛋白(CR)用于诊断先天性无神经节细胞症(HD)的可行性。方法采用免疫组织化学法检测CR在50例HD(HD扩张段组、HD痉挛段组)和10例对照组结肠壁的表达,并与常规HE染色进行对比分析。用Image-Pro-Plus图文分析系统定量分析染色结果。结果 HD痉挛段组与对照组及HD扩张段组黏膜下层、肌层间CR表达的平均光密度值(MOD)差异均有统计学意义(P<0.01);HE染色扩张段肠壁神经丛中可见神经节细胞,痉挛段神经节细胞缺失,CR免疫组织化学染色与HE染色诊断结果一致;新生儿与年长儿HD痉挛段黏膜下层CR表达差异无统计学意义(P=0.816)。结论 CR免疫组织化学染色用于新生儿HD的诊断与传统方法比较具有明显优越性。
Objective To investigate the feasibility of using calcium retinal protein (CR) in the diagnosis of idiopathic ganglioneuropathies (HD). Methods Immunohistochemistry was used to detect the expression of CR in colonic wall of 50 patients with HD (HD extended segment, HD Spastic segment) and 10 controls, and compared with conventional HE staining. The results of the staining were quantitatively analyzed by Image-Pro-Plus graphic analysis system. Results There were significant differences in the average optical density (MOD) values of CR expression between the HD spastic segment group and the control group and the HD extended segment group in the submucosa and muscular layer (P <0.01). HE staining in the dilated segment of the intestinal wall plexus Visible ganglion cells, spastic ganglion cells missing, CR immunohistochemical staining and HE staining diagnostic results; neonatal and elder children with HD spasm segment submucosa CR expression was no significant difference (P = 0.816). Conclusion CR immunohistochemical staining for the diagnosis of neonatal HD has obvious advantages compared with the traditional method.