目的:探索不同光质对桑黄菌丝生长及代谢产物累积变化规律。方法:分离、纯化、鉴定野生桑黄菌株;采用暗处理和白、蓝、绿、黄、红5种不同波长的光对桑黄菌丝进行照射培养,通过菌落直径、颜色变化及HPLC分析代谢产物累积变化和测定代谢产物对DPPH自由基清除活性变化,分析不同光质照射对桑黄菌丝生长及代谢产物累积影响。结果:分子生物学鉴定分离所得菌株为桑黄纤孔菌Inonotus sanghuang Sheng H.Wu,T Hatt.&Y.C.Dai。不同光质对该桑黄菌丝体生长有显著影响,其中暗培养、黄和红光处理生长速度及菌落形态上基本一致,但白、蓝和绿光处理生长抑制作用明显,菌丝看上去稀疏但菌落却厚实,并分泌大量黄色色素。HPLC分析结果表明,白、蓝和绿光处理20 d可显著增加代谢产物类型及丰度,其中苯乙烯基吡喃酮骨架类化合物Hypholomine B和Inoscavin A两个组分尤为显著,同时代谢产物对DPPH自由基清除活性也显著增加;蓝光处理后其胞内多糖却显著下降,而黄光处理则显著增加,其他处理组与暗培养组相当。不同光照处理时间各代谢产物HPLC图谱呈规律性变化,进一步说明光对桑黄代谢具有显著调控作用。结论:不同光质对桑黄生长、发育及代谢产物累积具有重要调控作用,暗培养有利于桑黄菌丝生长及提高生物量,蓝、绿光虽然对桑黄菌丝生长具有一定抑制作用,但可显著增加药效活性成分累积,因此,深入研究可为科学合理开发利用桑黄奠定基础。 Objective: To explore the different light quality of Phellinus igniarius growth and accumulation of metabolic changes. Methods: Isolation, purification and identification of wild Phellinus igniarius strains were carried out by dark treatment and light of 5 different wavelengths of white, blue, green, yellow and red. The diameter of colony, color change and HPLC Product accumulation changes and determination of metabolites scavenging DPPH free radical scavenging activity changes, analysis of different light quality of Phellinus igniarius growth and accumulation of metabolites. Results: The molecular biological identification of the isolated strain is Pseudomonas fluorescens Inonotus sanghuang Sheng H. Wu, T Hatt. & Y.C. Dai. Different light quality had significant effects on the growth of P. moris hyphae, of which, the growth rate of dark and yellow and red and the morphology of the colony were basically the same, but the growth inhibitory effects of white, blue and green were obvious, while the hyphae appeared sparse Colonies are thick, and the secretion of a large number of yellow pigment. The results of HPLC analysis showed that the metabolites types and abundances were significantly increased after treated with white, blue and green light for 20 days, of which the two components, styryl pyrone skeletons, Hypholomine B and Inoscavin A were especially significant, DPPH free radical scavenging activity was also significantly increased; the blue polysaccharide but a significant decrease in intracellular polysaccharide, while the yellow treatment was significantly increased, the other treatment group and dark culture group. The HPLC profiles of metabolites in different light treatment time showed regular changes, which further showed that light had a significant regulatory effect on the metabolism of Phellinus igniarius. CONCLUSION: Different light quality plays an important regulatory role on the growth, development and accumulation of metabolites of Phellinus igniarius. Dark culture is beneficial to the growth and biomass of Phellinus igniarius, although blue and green light may have some inhibitory effects on the growth of Phellinus igniarius, But can significantly increase the accumulation of pharmacologically active ingredients, therefore, in-depth study can lay the foundation for the scientific and rational development and utilization of Phellinus linteus.