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目的了解广州某部新兵连戊型病毒性肝炎(戊型肝炎)暴发流行的分子病毒学特征,并与当地散发毒株比较,以查找病原可能来源。方法用逆转录聚合酶链反应(RT-PCR)法,对抗HEV-IgM阳性的34例暴发性戊型肝炎及46例散发性戊型肝炎患者的血清和粪便标本进行HEVRNA检测,并对HEVRNA阳性标本的基因开放读码框(ORF)2部分片段进行克隆测序分析。结果34例暴发流行病例标本中检测出12株病毒,46例散发病例标本中检到2株。经克隆测序分析,各暴发毒株的核苷酸同源性为95.3%~100%;氨基酸同源性为94.0%~100%。且暴发毒株和散发毒株的核苷酸及氨基酸的同源性也较高,分别为95.3%~99.3%和94.0%~100%;暴发毒株和散发毒株与各型中的标准株相比,与Jap1株同源性最高,其核苷酸同源性为92.0%~95.3%,氨基酸同源性为96.0%~100.0%。进化树分析提示本次戊型肝炎暴发流行病毒株与戊型肝炎病毒基因Ⅳ型距离最近。结论本次戊型肝炎暴发流行的病原可能来于广州本地;广州地区戊型肝炎流行毒株属戊型肝炎基因型Ⅳ型。
Objective To understand the molecular virological characteristics of outbreaks of hepatitis E (Hepatitis E) in some recruits in Guangzhou and to compare with the locally distributed strains in order to find out the possible origin of pathogens. Methods HEV RNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR) in 34 cases of fulminant hepatitis E and 46 cases of sporadic hepatitis E in HEV-IgM. HEV RNA positive Specimens of gene open reading frame (ORF) 2 partial cloning and sequencing analysis. Results Of the 34 outbreak cases, 12 strains were detected in the epidemic and 2 strains were detected in 46 cases. According to the cloning and sequencing analysis, the nucleotide homology of each outbreak strain was 95.3% ~ 100%; the amino acid homology was 94.0% ~ 100%. The nucleotide and amino acid identities of the outbreak and sporadic strains were also higher, ranging from 95.3% to 99.3% and 94.0% to 100%, respectively. Compared with Jap1 strain, the homology was highest, with 92.0% -95.3% nucleotide homology and 96.0% -100.0% amino acid homology. Phylogenetic tree analysis suggested that the hepatitis E virus outbreak was closest to type IV of hepatitis E virus. Conclusions The etiological agent of the outbreak of hepatitis E may come from Guangzhou. Genotype E of hepatitis E is a type IV hepatitis E genotype in Guangzhou.