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目的探讨内毒素脑损伤模型大鼠的额叶皮质、海马组织、脑脊液(CSF)和血浆中脑红蛋白(NGB)的表达变化及其意义。方法将SD大鼠随机分为内毒素干预组和对照组,内毒素干预组向大鼠第四脑室内注射内毒素0.1mg/kg,对照组注入等剂量的生理盐水。于注射后3、6、12、24、48和72h采血,分离血清,收集CSF,并处死大鼠,留取额叶皮质和海马组织,应用ELISA、Western blot检测NGB含量,免疫组织化学法检测NGB的表达,干燥法检测鼠脑含水量。结果注射6h后,内毒素干扰组鼠脑含水量明显高于对照组,48h达峰值;额叶皮质、海马组织、CSF和血浆中NGB含量显著高于对照组,48h达峰值。结论在内毒素所致脑损伤中,NGB表达上调,且与内毒素的注入时间相关,NGB表达上调是机体内源性神经保护机制之一。
Objective To investigate the changes and significance of cerebrospinal fluid (CSF) and plasma neutrophil (NGB) in the rat model of endotoxin brain injury. Methods SD rats were randomly divided into endotoxin intervention group and control group. The endotoxin intervention group was injected endotoxin 0.1 mg / kg into the fourth ventricle of rats, and the control group was injected with normal saline. Serum samples were collected at 3, 6, 12, 24, 48 and 72 hours after injection. CSF was collected and rats were sacrificed. Prefrontal cortex and hippocampus tissue were removed. ELISA and Western blot were used to detect the content of NGB. Immunohistochemistry NGB expression, dry method to detect rat brain water content. Results After 6 hours of injection, the water content of brain tissue in endotoxin-treated rats was significantly higher than that in control rats, reaching a peak at 48 hours. The contents of NGB in frontal cortex, hippocampus, CSF and plasma were significantly higher than those in control group, reaching the peak at 48 hours. Conclusions NGB expression is upregulated in endotoxin-induced brain injury and is related to the time of endotoxin infusion. The upregulation of NGB is one of the mechanisms of endogenous neuroprotection in the body.