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目的 :研究三氧化二砷（ As2O3）对肝癌细胞株 Bel-7402的抑制增殖效应及凋亡诱导作用。方法 :采用 MTT法检测药物对细胞的抑制作用、相差显微镜和电镜观察细胞形态变化、琼脂糖凝胶电泳测定 DNA Ladder、流式细胞术检测细胞周期变化。结果 :各浓度 As2O3组（ 0.25～ 16μ mol/L）均可抑制肝癌细胞增殖，且抑制率具有浓度和时间依赖性，同作用时间呈直线相关，其中浓度组为 10μ mol/L抑制率最大， 96 h时达 83. 89％， 2μ mol/L As2O3以抑制肝癌细胞的增殖为主，但在形态及生化方面未见凋亡改变 ;10μ mol/L As2O3作用 10 h可见形态学变化， 48 h可见 DNA Ladder出现。流式细胞检查见明显的凋亡峰出现（ 8.66％），并使细胞周期阻滞在 S＋ G2 M期。结论：低浓度 As2O3（ 0.25～ 2.0μ mol/L）可以在体外抑制肝癌细胞的增殖，但未进入实质凋亡；高浓度 10μ mol/L组可诱导肝癌细胞凋亡。
Objective : To study the effect of arsenic trioxide (As2O3) on proliferation and apoptosis induction of hepatoma cell line Bel-7402. Methods: The inhibitory effect of drugs on the cells was detected by MTT assay, the cell morphology was observed by phase contrast microscope and electron microscope, the DNA Ladder was determined by agarose gel electrophoresis, and the cell cycle was detected by flow cytometry. RESULTS: As2O3 group (0.25-16μmol/L) could inhibit the proliferation of hepatocellular carcinoma cells in a concentration- and time-dependent manner. The time of action was linear, and the inhibitory rate of 10μmol/L was the highest in the concentration group. At 96 h, it reached 83.89%. 2μmol/L As2O3 mainly inhibited the proliferation of hepatocellular carcinoma cells, but no morphological and biochemical changes were observed. 10μmol/L As2O3 showed a morphological change at 10 h, 48 h. DNA Ladder appears. Flow cytometry showed a clear apoptotic peak (8.66%) and cell cycle arrest in the S+G2-M phase. Conclusion: Low concentration of As2O3 (0.25-2.0 μmol/L) can inhibit the proliferation of hepatoma cells in vitro, but does not enter into parenchymal apoptosis. Apoptosis of hepatocellular carcinoma cells can be induced by high concentration of 10 μmol/L.