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在巴西地方性原虫病中,恰加斯氏病和利什曼病十分常见。恰加斯氏病是由克氏锥虫感染所致;而利什曼原虫能引起皮肤、粘膜皮肤和内脏利什曼病且有双重感染的现象。用于诊断锥虫病的血清学方法,如间接免疫荧光试验(IFA)、间接血凝试验(IHA)和酶联免疫吸附试验(ELISA)容易与利什曼原虫感染后产生的抗体发生交叉反应,给流行病学资料的收集、整理和疾病的正确诊断、治疗带来一定的困难。本文作者对克氏锥虫粗抗原-IFA、IHA和ELISA及克氏锥虫特异性重组抗原(rTc24)-ELISA进行了比较,观察它们能否区分人体克氏锥虫和利什曼原虫感
Among endemic parasitic diseases in Brazil, Chagas disease and leishmaniasis are very common. Chagas disease is caused by Trypanosoma cruzi infection; Leishmania can cause skin, mucocutaneous and visceral leishmaniasis and double infection. Serological methods for the diagnosis of trypanosomiasis, such as indirect immunofluorescence (IFA), indirect hemagglutination (IHA) and enzyme-linked immunosorbent assay (ELISA), are prone to cross-react with antibodies produced after Leishmania infection , To epidemiological data collection, sorting and disease diagnosis, treatment brings some difficulties. The authors compared Trypanosoma cruzi crude antigens -IFA, IHA, and ELISA and Trypanosoma cruzi specific recombinant antigen (rTc24) -ELISA to determine whether they were able to differentiate between T. cruzi and Leishmania