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目的探讨我国沈阳地区汉族人群中转录因子7样2(Transcription factor 7-like 2,TCF7L2)基因rs290487-T/C单核苷酸多态性与2型糖尿病(Type 2 diabetes mellitus,T2DM)患者胰岛素抵抗的相关性。方法选择224例T2DM患者,提取其外周血基因组DNA,采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)对其TCF7L2基因rs290487位点进行基因分型,同时检测各项临床指标,并分析其与TCF7L2基因rs290487-T/C多态性的相关性。结果 T2DM患者组rs290487位点CT+CC基因型的胰岛素抵抗指数(Homeostasis model assessment of insulin resistance,HOMA-IR)、胰岛素β细胞功能指数(Homeostasis model assessment of Beta cell function index,HOMA-β)及空腹胰岛素(Fasting insulin,FINS)均高于TT基因型,差异有统计学意义(P<0.05)。结论沈阳地区汉族人群TCF7L2基因rs290487位点的多态性与T2DM患者胰岛素抵抗相关,为T2DM的病因学研究提供了新的思路。
Objective To investigate the relationship between rs290487-T / C single nucleotide polymorphism of transcription factor 7-like 2 (TCF7L2) gene and type 2 diabetes mellitus (T2DM) in Chinese Han population of Shenyang, Correlation of resistance. Methods A total of 224 patients with T2DM were selected and genomic DNA was extracted from peripheral blood. Genotypes of rs290487 TCF7L2 gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Indicators and analyzed its association with TCF7L2 rs290487-T / C polymorphism. Results Homeostasis model assessment of insulin resistance (HOMA-IR), Homeostasis model assessment of Beta cell function index (HOMA-β) and fasting Fasting insulin (FINS) was higher than TT genotype, the difference was statistically significant (P <0.05). Conclusion The rs290487 polymorphism of TCF7L2 gene in Han population of Shenyang is related to insulin resistance in T2DM patients, which provides a new idea for the etiological study of T2DM.