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目的建立高效液相色谱法测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量。方法色谱柱为CAPCELL PAK C18(150 mm×4.6 mm,5μm);流动相A为乙腈,B为水;流速:1.0 mL.min-1;检测波长为203 nm;洗脱程序:0~12 min,A相19%,B相81%;12~60 min,A为19%→36%,B为81%→64%。结果三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1保留时间分别为21,25,51 min,与各自相邻峰的分离度均在1.5以上。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.100 2~2.00 4μg、0.418 8~8.376μg和0.187~3.74μg。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为98.5%,99.6%和96.5%,RSD分别为1.9%,1.3%和1.9%。结论本法简便、准确、重现性好,可用于乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的同时测定。
Objective To establish a HPLC method for determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Ruyuxiao granules. Methods The column was CAPCELL PAK C18 (150 mm×4.6 mm, 5 μm). The mobile phase A was acetonitrile, B was water, the flow rate was 1.0 mL.min-1, the detection wavelength was 203 nm, and the elution procedure was 0-12 min. , A phase 19%, B phase 81%; 12 ~ 60 min, A is 19% → 36%, B is 81% → 64%. Results The retention time of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 21, 25, and 51 min, respectively, and their respective peaks were more than 1.5. The linear ranges of notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1 were 0.100 2 to 2.00 4 μg, 0.418 8 to 8.376 μg, and 0.187 to 3.74 μg, respectively. The recoveries of notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1 were 98.5%, 99.6%, and 96.5%, respectively, with RSDs of 1.9%, 1.3%, and 1.9%, respectively. Conclusion This method is simple, accurate and reproducible. It can be used for simultaneous determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Ruyuxiao Granules.