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用新生乳鼠心肌做原代细胞单层培养后均加入心得安(Propranolo)。分为4组:对照组;去甲肾上腺素(NE)组;NE+CEC组;NE+Nifedipine组。加入~3H-胸腺嘧啶核苷后测放射性计数。结果表明,CEC阻断α_(1b)亚型受体后,不影响NE刺激心肌细胞DNA的合成;Nifedpine阻断α_(1a)亚型受体后,完全阻断了NE的作用。提示,α_(1a)亚型受体可能是交感-儿茶酚胺刺激心肌肥大形成的机理之一。
Propranolol was added into primary cell monolayers of neonatal rat cardiomyocytes. Divided into 4 groups: control group; norepinephrine (NE) group; NE + CEC group; NE + Nifedipine group. Radioactive counts were measured after adding ~ 3H-thymidine. The results showed that CEC blocked α1b receptor did not affect the DNA synthesis of cardiomyocytes induced by NE. Nifedpine completely blocked the effect of NE after blocking α1a receptor. It is suggested that αα (1a) subtype receptor may be one of the mechanisms of sympathetic-catecholamine stimulation of cardiac hypertrophy.