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目的:通过分析不同浓度NaCl胁迫下甘草生长和抗氧化酶活性的变化探讨甘草对盐分胁迫的适应性机理;方法:采用不同浓度的NaCl溶液(配成Hoagland液)处理盆栽一年生甘草,分别于35d、70d和105d取样,测定甘草株高、地上部分鲜、干重、根鲜、干重及甘草叶片SOD、POD、CAT活性,分析各生长指标与抗氧化酶活性的相关性;结果:NaCl胁迫70d和105d,0.6%和0.9%处理组的株高、地上部分鲜、干重及根鲜、干重均显著低于CK,SOD、POD及CAT活性均显著高于CK,经相关性分析得知,SOD、POD及CAT活性与各生长指标均负相关,其中POD活性与各生长指标极显著负相关;结论:甘草对NaCl胁迫的响应有胁迫时间和浓度的依赖性,在遭遇胁迫时,通过改变自身的生长和提高抗氧化酶活性,来提高机体的抗盐能力。
Objective: To investigate the adaptive mechanism of licorice to salt stress by analyzing the changes of growth and antioxidant enzyme activity of licorice under different concentrations of NaCl stress.Methods: The annual licorice was treated with different concentrations of NaCl solution (dubbed Hoagland fluid) , 70d and 105d samples were collected to determine the SOD, POD and CAT activities of plant height, above-ground fresh, dry weight, root fresh weight, dry weight and licorice leaves. The correlation between each growth index and antioxidant enzyme activity was analyzed. Results: The plant height, shoot fresh weight, dry weight, root fresh weight and dry weight at 70d and 105d, 0.6% and 0.9% treatments were significantly lower than CK, and the activities of SOD, POD and CAT were significantly higher than CK The activities of SOD, POD and CAT were negatively correlated with each growth index, of which POD activity was negatively correlated with each growth index. Conclusion: The response of Glycyrrhiza uralensis to NaCl stress was dependent on the time and concentration of stress, By changing their own growth and improve antioxidant enzyme activity, to improve the body’s salt resistance.