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一氧化氮(NO)和活性氧(ROS)是植物体内两种常见的信号分子,在植物抗逆反应等过程中起重要作用.NO合成和氧化迸发(oxidativeburst)以及ROS积累是红豆杉悬浮细胞在桔青霉细胞壁诱导子处理下出现的两个早期反应.为了探讨NO和ROS在桔青霉细胞壁诱导子促进红豆杉细胞紫杉醇合成过程中的作用及其相互关系,分别以NO专一性淬灭剂cPITO,一氧化氮合酶(NOS)抑制剂PBITU,质膜NAD(P)H氧化酶抑制剂DPI以及超氧离子(O2)和过氧化氢(H2O2)淬灭剂超氧化物歧化酶(SOD)及过氧化?氢酶(CAT)处理红豆杉细胞.结果表明,cPITO和DPI不仅可以分别抑制红豆杉细胞的NO合成和ROS积累,同时还可以阻断诱导子对紫杉醇合成的促进作用,说明NO和ROS是参与桔青霉细胞壁诱导子促进红豆杉细胞中紫杉醇合成调控的信号分子.cPITO和PBITU同时还可以部分抑制诱导子对红豆杉细胞氧化迸发的诱发作用.外源NO单独处理可以促进红豆杉细胞中紫杉醇合成,DPI可以抑制NO对紫杉醇合成促进作用.然而,即使在红豆杉细胞中ROS积累被完全抑制的情况下,NO和桔青霉细胞壁诱导子对细胞中紫杉醇的合成仍然具有一定的促进作用.上述结果表明,NO可以通过依赖和不依赖ROS的两类不同信号途径介导真菌诱导子诱发红豆杉细胞中紫杉醇的生物合成.实验结果同时也表明,NO和桔青霉细胞壁诱导子对红豆杉细胞中紫杉醇合成的促进作用可以被CAT抑制,但不受SOD的影响,说明氧化迸发产生的H2O2可能是介导NO和桔青霉细胞壁诱导子诱发紫杉醇合成的信号分子.
Nitric oxide (NO) and reactive oxygen species (ROS) are two common signaling molecules in plants and play an important role in plant anti-retrogradation reactions, etc. NO synthesis and oxidative bursting and ROS accumulation are two main signaling pathways Two early reactions of Penicillium citricum cell wall inducer treatment.In order to investigate the role of NO and ROS in the induction of paclitaxel in Taxus cell walls induced by Penicillium citrinum cell wall and their correlation with NO specific quenching CPITO, PBITU, a nitric oxide synthase (NOS) inhibitor, DPI, a plasma membrane NAD (P) H oxidase inhibitor, and superoxide dismutase (O2) and hydrogen peroxide SOD and CAT. The results showed that cPITO and DPI could not only inhibit the NO synthesis and ROS accumulation of Taxus cuspidata cells, but also inhibit the promotion of paclitaxel synthesis by elicitors, These results suggest that NO and ROS are involved in the induction of paclitaxel synthesis in Taxus cuspidata cell wall inducer and that cPITO and PBITU can partially inhibit the inducing effect of elicitors on the oxidative burst of Taxus cell.Exogenous NO alone Can promote the paclitaxel synthesis in Taxus cells and DPI can inhibit the promotion of paclitaxel production by NO.However, even though ROS accumulation is completely inhibited in Taxus cells, the synthesis of paclitaxel by NO and Penicillium citricum cell wall inducer Still play some role in promoting the growth of Taxus chinensis.The above results indicate that NO can induce paclitaxel biosynthesis in Taxus cuspidata cells through two different signaling pathways dependent on and not dependent on ROS.The results also show that NO and orange green The promoting effect of mold cell wall elicitor on paclitaxel synthesis in Taxus cell can be inhibited by CAT but not by SOD, indicating that H2O2 produced by oxidative burst may be the signaling molecule that mediates the induction of paclitaxel synthesis by NO and Penicillium citricum cell wall inducer .