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在美国饮用水中已鉴定出300余种化学物质,作者选其中的71种,用Ames的组氨酸缺陷型鼠伤寒沙门氏菌平板渗入法检测其致突变性。对于挥发性物质则采用干燥器法或悬浊液法。用多氯联苯诱导大鼠或直接用小鼠和人的肝匀浆作为化学物质的体外激活系统。此外,还用埃希氏大肠菌WP_2或麦酒酵母D_3检测某些化学物质的致突变作用。结果表明,有45种化学物质未能引起鼠伤寒沙门氏菌的回复突变。另外的26种化学物质在一种或多种检测系统中均引起微生物的回复突变,见表。
More than 300 chemicals have been identified in drinking water in the United States of America, of which 71 were selected for their mutagenicity by Ames histidine-defective Salmonella typhimurium plate infiltration. For the volatile substances are used dryer method or suspension method. In vitro activation of polyclonal-induced rat or mouse and human liver homogenates as chemicals. In addition, Escherichia coli WP_2 or S. cerevisiae D_3 were also used to detect the mutagenicity of some chemicals. The results showed that 45 kinds of chemical substances failed to cause the Salmonella typhimurium back-mutation. The other 26 chemicals cause microbial reversion in one or more detection systems, as shown in the table.