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我们在工作中时常发现胶乳凝集试验阳性结果与临床症状和相关检查不符。为寻找其中的原因,作者偶然发现1:100新洁尔灭消毒液对胶乳凝集试验有一定的干扰,特设计实验加以证实。现报道如下。 1.材料与方法 采用美国ABI公司提供的绒毛膜促性腺激素(HCG)和上海医学化验所提供的抗链球菌溶血素“O”(ASO),类风湿因子(RF),C反应蛋白(CRP)四种试剂盒,均按试剂说明书操作,并以1:50—1:1000不同浓度的新洁尔灭为受检物进行重复检测。
We often find in work at the latex agglutination test positive results and clinical symptoms and related tests do not match. In order to find out the reason, the author accidentally discovered 1: 100 benzalkonium bromide disinfectant latex agglutination test has some interference, special design experiments to be confirmed. Report as follows now. 1. Materials and Methods The human chorionic gonadotropin (HCG) provided by ABI and the anti-streptolysin O (ASO), rheumatoid factor (RF) and C-reactive protein (CRP) ) Four kits were operated according to the instruction manual of the reagent, and repeated tests were carried out with benzalkonium chloride of 1: 50-1: 1000 in different concentrations.