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本文首次报道了应用山茶花嫩叶圆片作为诱饵分离土壤中樟疫霉的技术。山茶花嫩叶圆片(直径9mm)3～5片漂浮于20g 土:80ml 蒸馏水的水面上,在光暗交替和自然变温条件下,诱集3d～4d,再于选择性培养基上培养3d～4d,可得到最佳的分离效果。它比雪松、杜鹃、柑桔和梨等材料作为诱饵的分离诱集率高,速生杂菌污染率低,取材也较方便。此种方法为土传病害疫病菌,尤其是樟疫霉的分离、生态等研究提供了有效的途径。
This paper reports for the first time the technique of using camellia bulbs as bait to separate P. camphora in soil. 3 to 5 pieces of camellia leaf discs (9mm in diameter) are floated on the water surface of 20g soil: 80ml of distilled water, and are trapped for 3d to 4d under alternating dark and light conditions and natural temperature, and then cultured on selective medium for 3days, 4d, get the best separation effect. It is higher than cedar, rhododendron, citrus and pear materials as bait separation and entrapment rate is high, quick-contamination rate of bacteria is low, drawing is more convenient. This method provides an effective way for the research on the pathogens of Phytophthora infestans, especially the separation and ecology of P. cinnamomi.