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颗粒细胞造釉细胞瘤很少见,占所有造釉细胞瘤的5%。这种颗粒细胞单个或呈团,部分或完全占据滤泡的中心区。一些超微结构和组织化学研究,认为这种颗粒为溶酶体。本文作者用电镜和组织化学的研究方法,以便弄清这些颗粒的组织学来源。材料和方法 4例颗粒细胞造釉细胞瘤的临床资料见表。肿瘤组织用10%福尔马林固定,常规组织学检查,切片用HE、PAS、阿新兰和油红O(脂肪染料)染色。作组织化学研究的两例,组织用冷甲醛钙液固定,后置于冷树胶蔗糖内过夜,低恒温切片。研究以下几种酶活性:酸性磷酸
Granulocyte ameloblastoma is rare and accounts for 5% of all ameloblastomas. The granulosa cells, either alone or in clumps, partially or completely occupy the central area of the follicles. Some ultrastructural and histochemical studies suggest that the particles are lysosomes. The authors used electron microscopy and histochemical methods to understand the histological origin of these particles. Materials and Methods The clinical data of 4 cases of granulosa cell ameloblastoma are shown in the table. Tumor tissues were fixed with 10% formalin and routine histological examinations. Sections were stained with HE, PAS, ANZ and oil red O (fat dye). For two cases of histochemical studies, the tissue was fixed with cold formalin calcium, placed overnight in cold gum sucrose, and sectioned at low temperature. The following enzyme activities are studied: Acidic Phosphoric Acid