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为研究低强度Ｈｅ－Ｎｅ激光照射对离体小鼠巨噬细胞内Ｃａ２＋浓度的影响以及其与能量密度（剂量）的关系，用Ｆｌｕｏ－３乙酰甲脂对巨噬细胞Ｃａ２＋作荧光标记，应用图象分析系统检测激光照射后细胞内Ｃａ２＋的荧光强度变化。结果：Ｈｅ－Ｎｅ激光照射引起细胞内Ｃａ２＋浓度改变。照射５ｍｉｎ，激光剂量为０．６７９Ｊ／ｃｍ２，细胞荧光强度出现非常明显的增强；照射１０ｍｉｎ，剂量为１．３５８Ｊ／ｃｍ２，细胞荧光强度增强达到最大值；照射１５ｍｉｎ，剂量为２．０３７Ｊ／ｃｍ２，细胞荧光强度减弱；照射２０ｍｉｎ，剂量为２．７１６Ｊ／ｃｍ２，细胞荧光强度降低至对照组之下。低强度Ｈｅ－Ｎｅ激光照射巨噬细胞可引起细胞内Ｃａ２＋浓度上升，产生细胞内信号传递，进而控制细胞功能。这可作为临床上应用低强度激光照射治疗的参考
In order to investigate the effect of low-intensity He-Ne laser irradiation on intracellular Ca2 + concentration in murine macrophages and its relationship to energy density (dose), fluorescent labeling of Ca2 + in macrophages was performed with Fluo-3 acetylcysteine Image analysis system detects changes in intracellular fluorescence intensity of Ca2 + after laser irradiation. Results: He-Ne laser irradiation caused changes of intracellular Ca2 + concentration. After 5 min irradiation, the laser intensity reached 0.679 J / cm 2, and the fluorescence intensity of the cells increased obviously. After irradiation for 10 min, the dose reached 1.358 J / cm 2, , The cell fluorescence intensity weakened; irradiation 20min, a dose of 2.716J / cm2, cell fluorescence intensity decreased below the control group. Low-intensity He-Ne laser irradiation of macrophages can cause intracellular Ca2 + concentration increases, resulting in intracellular signaling, and thus control cell function. This can be used as a clinical reference to the treatment of low-intensity laser irradiation