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目的:建立复方8-甲氧补骨脂素洗剂中复方8-甲氧补骨脂素和醋酸肤轻松含量测定的高效液相色谱法。方法:采用Waters Symmetry C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(70:30),检测波长为240 nm,流速为1.0 ml·min~(-1)。结果:8-甲氧补骨脂素浓度在20.232～101.16 mg·L~(-1)范围内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为100.70%(RSD为0.82%)。醋酸肤轻松浓度在5.232—26.16 mg·L~(-1)范围内与峰面积呈良好的线性关系(r=0.999 8),平均回收率为100.8%(RSD为1.11%)。结论:本法简便、快捷,结果准确,适用于该制剂的质量分析检验。
OBJECTIVE: To establish a HPLC method for the determination of the content of 8-methoxy psoralen and triacetin in compound 8-methoxy psoralen lotion. Methods: The mobile phase was methanol - water (70:30) with a detection wavelength of 240 nm and a flow rate of 1.0 ml · min -1 using a Waters Symmetry C 18 column (250 mm × 4.6 mm, 5 μm) . Results: The concentration of 8-methoxypsoralen showed a good linear relationship with the peak area (r = 0.999 9) in the range of 20.232 ~ 101.16 mg · L -1 with an average recovery of 100.70% (RSD 0.82 %). The results showed that there was a good linear relationship (r = 0.999 8) with the average apparent recovery of 100.8% (RSD 1.11%) in the range of 5.232-26.16 mg · L -1. Conclusion: This method is simple, rapid, accurate and suitable for the quality analysis of the preparation.