【摘 要】
：
背景:脐带Whartons Jelly中间充质干细胞可以向胰岛样细胞诱导分化.目的:验证脐带源间充质干细胞与大鼠胰腺细胞共培养向胰岛样细胞诱导分化的可能性,并观察移植后对糖尿病大
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背景:脐带Whartons Jelly中间充质干细胞可以向胰岛样细胞诱导分化.目的:验证脐带源间充质干细胞与大鼠胰腺细胞共培养向胰岛样细胞诱导分化的可能性,并观察移植后对糖尿病大鼠血糖的影响.方法:分离、诱导、传代脐带Whartons Jelly中间充质干细胞,再与大鼠胰腺细胞共培养,诱导成胰岛细胞团样组织.将大鼠分为3组,正常对照组不进行移植及造模;模型组仅制备糖尿病大鼠模型;实验组造模后将胰岛样细胞移植入糖尿病大鼠肾脏包膜.结果与结论:脐带Whartons Jelly细胞培养中有细胞从组织块中爬出,第7天形态发生变化,贴壁细胞部分变成梭形.分离培养的细胞表达具有间充质干细胞表面特有标志CD44、CD29、CD105,不表达CD34、CD45、CD14.诱导第7,10天PDX-1及人胰岛素强染色;胰岛素及C-肽浓度较单纯培养组明显升高;PDX-1及人胰岛素mRNA诱导第7、10天较高表达.移植第1周大鼠尾尖血糖链脲佐菌素实验组明显低于模型组(P < 0.01),但明显高于正常照组(P < 0.01).8周链脲佐菌素实验组肾脏被膜下发现胞核染棕色染色的Brdu阳性、胞浆棕色染色的胰岛素阳性细胞.结果表明,脐带Whartons Jelly中存在脐带源间充质干细胞,与大鼠胰腺细胞共培养可促进间充质干细胞向胰岛样细胞诱导分化,移植入糖尿病大鼠肾脏被膜下,可显著降低糖尿病大鼠血糖.
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