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目的：探讨淋巴细胞功能相关抗原－１（ＬＦＡ－１）对活动性狼疮肾炎（ＬＮ）外周血单个核细胞（ＰＢＭＣ）白介素 １０（ＩＬ－１０）的调节作用。方法：利用半定量 ＲＴ－ＰＣＲ技术观察 ＬＦＡ－ １共刺激对活动性 ＬＮ患者 ＰＢＭＣ ＩＬ－１０ ｍＲＮＡ表达的影响。结果：单独抗ＣＤ３抗体（３０ ｎｇ／ｍｌ）能诱导ＬＮ 患者ＰＢＭＣ ＩＬ－１０ ｍＲＮＡ轻微表达（０．４３＋０．０３ｖｓ ０．５５±０．４４，Ｐ＜０．０５）而在抗ＣＤ３抗体（３０ ｎｇ／ｍｌ）存在的情况下，抗ＬＦＡ－１抗体（２ μｇ／ｍｌ，相当于ＬＦＡ－１配体）共刺激时明显增加了ＰＢＭＣ ＩＬ—１０ ｍＲＮＡ表达（１．３１±０．０８ｖｓ ０．５５＋０，０４，Ｐ＜０．０１）。ＬＦＡ－１中和抗体明显抑制了这种共刺激诱导的ＩＬ—１０ ｍＲＮＡ表达（１．３１±０．０８ｖｓ ０．６４ ±０．０３，Ｐ＜０．０１）。抗ＬＦＡ－１抗体单独刺激时并不引起 ＩＬ－１０ ｍＲＮＡ表达变化（Ｐ＞０．０５）。结论：ＬＦＡ－１作为共刺激分子诱导 ＩＬ－ １０ ｍＲＮＡ表达可能是其参与 ＬＮ发病的机制之一。
Objective: To investigate the regulatory effect of LFA-1 on interleukin-10 (IL-10) in peripheral blood mononuclear cells (PBMC) of patients with active lupus nephritis (LN). Methods: The effects of LFA-1 co-stimulation on IL-10 mRNA expression in PBMC of patients with active LN were observed by semi-quantitative RT-PCR. Results: The anti-CD3 antibody (30 ng / ml) induced a slight expression of IL-10 mRNA in PBMC of LN patients (0.43 + 0.03 vs 0.55 ± 0.44, P <0.05) IL-10 mRNA expression was significantly increased in PBMCs (1.31 ± 0.08 vs. 0) when co-stimulated with anti-LFA-1 antibody (2 μg / ml, equivalent to LFA-1 ligand) 55 + 0,04, P <0.01). Neutralizing antibody to LFA-1 significantly inhibited this costimulation-induced IL-10 mRNA expression (1.31 ± 0.08 vs. 0.64 ± 0.03, P <0.01). Anti-LFA-1 antibody alone did not induce changes in IL-10 mRNA expression (P> 0.05). Conclusion: LFA-1 as costimulatory molecule induces IL-10 mRNA expression may be one of the mechanisms involved in the pathogenesis of LN.