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目的观察低氧环境对子宫内膜异位症(EMs)的在位细胞葡萄糖、乳酸及三磷酸腺苷(ATP)的改变,探讨低氧在EMs发病机理中的作用。方法收集10例EMs患者在位内膜(A组)和10例正常内膜组织(B组),分别置于常氧(21%O2)与低氧(1%O2)培养箱中48h,测定葡萄糖消耗、乳酸及ATP的生成。结果常氧下,两组细胞葡萄糖消耗、乳酸及ATP生成水平相似(P>0.05)。低氧下,A组细胞葡萄糖消耗和乳酸生成均高于B组[(14.58±3.59)mmol·L-1·10-6cell vs.(8.58±5.54)mmol·L-1·10-6cell和(0.99±0.19)mmol·L-1·10-6cell vs.(0.58±0.29)mmol·L-1·10-6cell](P<0.05),而ATP生成水平相仿[(8.72±0.35)nmol×106cell vs.(8.70±0.53)nmol×106cell)(P>0.05)]。结论低氧可能提高EMs在位内膜的葡萄糖消耗及乳酸生成,从而可能有利于EMs的疾病发展。
Objective To observe the changes of glucose, lactate and adenosine triphosphate (ATP) in esophagus of endometriosis (EMs) in hypoxic environment and to explore the role of hypoxia in the pathogenesis of EMs. Methods Eutopic endometrium (group A) and normal endomembrane tissue (group B) were collected from 10 patients with EMs. The patients were placed in normoxia (21% O2) and hypoxia (1% O2) incubators for 48 hours, Depletion of glucose, production of lactic acid and ATP. Results Under normoxia, glucose consumption, lactate and ATP production were similar in both groups (P> 0.05). Under hypoxia, the glucose consumption and lactate production in group A were higher than those in group B ([(14.58 ± 3.59) mmol·L -1 .10-6cell vs. (8.58 ± 5.54) mmol·L -1 .10-6cell and ( 0.99 ± 0.19 mmol·L-1 · 10-6cell vs. 0.58 ± 0.29 mmol·L-1 · 10-6cell] (P <0.05), while the ATP production levels were similar [(8.72 ± 0.35) nmol × 106cell vs. (8.70 ± 0.53) nmol × 106cell) (P> 0.05)]. Conclusions Hypoxia may increase the glucose consumption and lactate production in the eutopic endometrium of EMs, which may be beneficial to the disease development of EMs.