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In vitro antioxidant activities of resveratrol and piceid against peroxynitrite(ONOO-) were examined by the inhibition of 3-nitrotyrosine formation.Trolox was used as a positive control.Resveratrol and piceid exhibited high ONOO--scavenging activities in a concentration dependent manner.The antioxidant activities(the concentration of test compound required to yield a 50% inhibition of tyrosine nitration,IC 50) of resveratrol and piceid against ONOO-were(48.34±0.97) and(74.69±1.49) μmol/L,respectively.Compared with that of trolox[(105.40±1.16) μmol/L],their scavenging activities were 2.2-and 1.5-fold higher for resveratrol and piceid.Formation of nitroresveratrol as shown by UV-Vis spectroscopy and liquid chromatography-tandom mass spectrometry(LC-MS/MS) analysis indicates that resveratrol could directly scavenge ONOO-via nitration reaction.Our results demonstrate that foods and medicinal herbs with resveratrol and piceid as stronger ONOO-scavengers are valuable ingredients and have healthy application in preventing humans from peroxynitrite-mediated oxidative damage by scavenging peroxynitrite efficiently.
In vitro antioxidant activities of resveratrol and piceid against peroxynitrite (ONOO-) were examined by the inhibition of 3-nitrotyrosine formation. Trolox was used as a positive control. Resveratrol and piceid exhibited high ONOO - scavenging activities in a concentration dependent manner. antioxidant activities (the concentration of test compound required to yield a 50% inhibition of tyrosine nitration, IC 50) of resveratrol and piceid against ONOO-were (48.34 ± 0.97) and (74.69 ± 1.49) μmol / L, respectively. Compared with that of trolox [(105.40 ± 1.16) μmol / L], their scavenging activities were 2.2-and 1.5-fold higher for resveratrol and piceid. Formation of nitroresveratrol as shown by UV-Vis spectroscopy and liquid chromatography-mass spectrometry (LC- MS / MS) analysis indicates that resveratrol could direct scavenge ONOO-via nitration reaction. Our results demonstrate that foods and medicinal herbs with resveratrol and piceid as stronger ONOO-scavengers are valuable ingredients and hav e healthy application in preventing humans from peroxynitrite-mediated oxidative damage by scavenging peroxynitrite efficiently.