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目的:观察megsin基因转染对高糖环境中肾小球系膜细胞单核细胞趋化蛋白-1(MCP-1)及细胞间黏附分子-1(ICAM-1)表达的影响。方法:高糖环境中培养小鼠肾小球系膜细胞,分别培养12、24、48 h,采用MTT法检测细胞增殖程度,免疫细胞化学和Western blot法检测系膜细胞megsin、MCP-1、ICAM-1蛋白表达水平,ELISA检测细胞培养上清Ⅳ型胶原浓度。结果:高糖环境中肾小球系膜细胞megsin、MCP-1及ICAM-1表达增强,细胞增殖明显,细胞上清液中Ⅳ型胶原浓度升高,megsin基因转染后上述变化趋势更加显著,而megsin shRNA质粒转染可明显减弱上述变化。结论:Megsin可上调MCP-1及ICAM-1表达,促进系膜细胞增殖及系膜外基质积聚。
Objective: To investigate the effect of megsin gene transfection on the expression of monocyte chemotactic protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) in mesangial cells in high glucose environment. METHODS: Mouse mesangial cells were cultured in high glucose for 12, 24 and 48 h respectively. MTT assay was used to detect the proliferation of mesangial cells. The expressions of megsin, MCP-1, ICAM-1 protein expression level, ELISA detection of cell culture supernatant type Ⅳ collagen concentration. Results: The expression of megsin, MCP-1 and ICAM-1 in glomerular mesangial cells was increased in high glucose and the cell proliferation was obvious. The concentration of type Ⅳ collagen in the supernatant of cells was increased. The above trend was more obvious after megsin gene transfection , While megsin shRNA plasmid transfection can significantly reduce the above changes. Conclusion: Megsin can up-regulate the expression of MCP-1 and ICAM-1, promote the proliferation of mesangial cells and the accumulation of extra-mesangial matrix.