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为建立测定人血清中美罗培南浓度的高效毛细管电泳方法,用乙腈(1:1 v/v)沉淀血清中的蛋白质,分离介质为66 mmol·L~(-1)磷酸盐缓冲液(pH=5.8);正极压力进样,进样压力50 mbar×10 s;分离电压30kV,柱温25℃,检测波长297 nm。结果表明,美罗培南与血清杂质无干扰,以头孢哌酮为内标,在5～200 mg·L~(-1)范围内其峰面积与内标峰面积的比值和浓度呈良好的线性关系;方法回收率98.4%～101.42%;日内和日间测定 RSD 分别小于8%和14%;最低检测浓度为4 mg·L~(-1)。结论:本方法简便、快速、准确,适用于血药浓度监测及临床药代动力学研究。
In order to establish a high performance capillary electrophoresis method for the determination of meropenem in human serum, the protein in serum was precipitated with acetonitrile (1: 1 v / v) and the separation medium was 66 mmol·L -1 phosphate buffer (pH = 5.8); positive pressure injection, injection pressure 50 mbar × 10 s; separation voltage 30kV, column temperature 25 ℃, detection wavelength 297 nm. The results showed that Meropenem had no interference with serum impurities, with cefoperazone as an internal standard, and its peak area and internal standard peak area ratio showed a good linear relationship with concentration in the range of 5 ~ 200 mg · L ~ (-1) The recoveries were 98.4% -101.42%. The RSDs were less than 8% and 14%, respectively. The minimum detectable concentration was 4 mg · L -1. Conclusion: The method is simple, rapid and accurate and is suitable for monitoring blood concentration and clinical pharmacokinetics.