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目的了解高强度超声聚焦热疗(HIFU)治疗后原发性肝癌患者的免疫功能变化情况。方法应用FEP BY01型肿瘤超声治疗机对42例原发性肝癌患者进行治疗。分别取HIFU治疗前和HIFU治疗后4周外周血10ml,检测T细胞亚群:CD4、CD8、CD4/CD8;Th细胞因子:IFNγ、IL2、IL4、Il10。结果42例肝癌患者治疗前和治疗后CD4、CD8T细胞百分数、CD4/CD8比值分别为(29.5±4.8)%和(38.2±5.1)%(P<0.05);30.2±6.4%和24.4±3.7%(P<0.05);0.9±0.3和1.7±0.2(P<0.01);治疗前和治疗后IL2、IFNγ、IL4、IL10含量(pg/ml)分别为93.5±28.3和110.5±24.2(P<0.05);73.2±18.6和92.8±20.1(P<0.05);82.3±14.2和60.2±11.3(P<0.01);74.8±21.9和56.2±19.1(P<0.01)。结论经HIFU治疗后肝癌患者的CD4T细胞百分数和CD4/CD8比值明显升高,CD8T细胞百分数下降;IL2和IFNγ水平明显升高,而IL4和IL10水平明显下降。应用HIFU技术治疗原发性肝癌可以改善患者体内的细胞免疫功能和Th1向Th2漂移的状态。
Objective To investigate the changes of immune function in patients with primary liver cancer after high intensity ultrasound focused hyperthermia (HIFU). Methods Forty-two patients with primary liver cancer were treated with FEP BY01 tumor ultrasonic therapy machine. The levels of CD4 + CD8, CD4 / CD8 and Th cytokines IFNγ, IL2, IL4 and Il10 were measured before and after treatment with HIFU for 4 weeks. Results The percentages of CD4 and CD8 T cells and CD4 / CD8 in 42 HCC patients before treatment and after treatment were (29.5 ± 4.8)% and (38.2 ± 5.1)% (30.2 ± 6.4%) and 24.4 ± 3.7% (P <0.05); 0.9 ± 0.3 and 1.7 ± 0.2 (P <0.01); The levels of IL2, IFNγ, IL4 and IL10 before and after treatment were 93.5 ± 28.3 and 110.5 ± 24.2 ; 73.2 ± 18.6 and 92.8 ± 20.1 (P <0.05); 82.3 ± 14.2 and 60.2 ± 11.3 (P <0.01); 74.8 ± 21.9 and 56.2 ± 19.1 (P <0.01), respectively. Conclusions The percentages of CD4 T cells and CD4 / CD8 in HCC patients after HIFU treatment are significantly higher, while the percentage of CD8 T cells is decreased. The levels of IL2 and IFN γ are significantly increased, while the levels of IL4 and IL10 are significantly decreased. The application of HIFU in the treatment of primary liver cancer can improve the cellular immune function and the state of Th1 to Th2 shift in patients.