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目的 :在先前已建立了模拟人艾滋病感染者和患者的猴免疫缺陷病毒 (SIV)急性感染猴和中、晚期猴艾滋病 (SAIDS)模型的基础上 ,再摸索建立SIV慢性感染猴模型 ,以期为抗艾滋病药物的体内药效学评价研究提供更合适的动物模型。方法 :用SIVmac2 51感染恒河猴 17只 ,追踪观察其临床表现 ,体征变化 ,病程进展 ,血浆和全血病毒血症规律 ,淋巴细胞亚群CD4 的动态等。结果 :临床体征有特征性 ,病程较长 ,1年内死亡 2只 ( 11.8% )。血浆病毒血症水平在感染后 14d达高峰 ,感染 2 1d开始下降 ,60～ 360d检不出或低滴度。全血病毒血症水平在感染后 10d开始持续高滴度至 3个月 ,3个月后仍有半数以上持续高滴度 ,9个月后才下降。淋巴细胞亚群CD4 值感染后 14～ 30d略下降 ,后回升。结论 :SIV慢性感染猴模型临床表现、病毒学和免疫学的变化及病程进展类似人艾滋病感染者在进入艾滋病中、晚期前的慢性感染过程 ,可用于抗艾滋病药物疗效的研究
OBJECTIVE: On the basis of the previously established monkey simian immunodeficiency virus (SIV) acute infection monkeys and middle and late stage monkey AIDS (SAIDS) models that simulate human AIDS patients and patients, we further explored the establishment of chronic infection monkey model of SIV with a view to In vivo pharmacodynamic evaluation of anti-AIDS drugs provides a more appropriate animal model. Methods: Seventeen rhesus monkeys were infected with SIVmac2 51 and their clinical manifestations, signs and symptoms, progression of the disease, the regularity of plasma and whole blood viremia, and the dynamic changes of CD4 in lymphocyte subsets were observed. Results: The clinical signs were characteristic, longer duration, 2 deaths within one year (11.8%). The level of plasma viremia peaked on day 14 after infection, and began to decline after 21 days of infection. No detectable or low titer was detected in 60 to 360 days. The level of whole blood viraemia continued high titers to 3 months after 10 days of infection and more than half of those with high titers remained after 3 months and declined only after 9 months. The CD4 level of lymphocyte subsets slightly decreased from 14 to 30 days after infection, and then rose. CONCLUSIONS: The clinical manifestations, virological and immunological changes and the course of disease in the chronic infection monkey model of SIV are similar to those of people living with HIV who are in the middle and advanced stages of AIDS, and can be used in the study of anti-AIDS drug efficacy