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目的通过检测子宫肌瘤组织及正常子宫平滑肌组织中增殖细胞核抗原(PC N A)和凋亡调控基因bcl-2的表达,探讨细胞增殖和细胞凋亡在子宫肌瘤发病机制中的作用。方法用免疫组织化学ABC法检测40例(增生期22例,分泌期18例)子宫肌瘤组织和正常子宫平滑肌组织中的PC N A和bcl-2蛋白的表达。结果PC N A和bcl-2在肌瘤组织中表达均强于正常子宫平滑肌组织,且分泌期强于增生期(P<0.01)。但在正常子宫平滑肌组织中两者增生期和分泌期的表达则无显著性差异(P>0.05)。结论PC N A和bcl-2表达的增加可能在子宫肌瘤的肿瘤发生过程中起重要作用。子宫肌瘤的发生不仅与肌瘤细胞增殖活性增高有关,而且与肌瘤细胞的凋亡受抑制有关。这种作用可能受到孕激素的调节。
Objective To investigate the role of cell proliferation and apoptosis in the pathogenesis of uterine fibroids by detecting the expression of proliferating cell nuclear antigen (PCNA) and apoptosis-regulating gene bcl-2 in uterine fibroid tissue and normal uterine smooth muscle. Methods The expressions of PCNA and bcl-2 protein in 40 cases of uterine myoma and normal uterine smooth muscle tissues were detected by immunohistochemical ABC method in 22 cases of proliferative phase and 18 cases of secretory phase. Results The expression of PCNA and bcl-2 in myoma was stronger than that in normal uterine smooth muscle, and the secretion was stronger than that in proliferative phase (P <0.01). However, in normal uterine smooth muscle tissue, there was no significant difference in proliferative phase and secretory phase (P> 0.05). Conclusion The increased expression of PC N A and bcl-2 may play an important role in the tumorigenesis of uterine fibroids. Uterine fibroids not only with the occurrence of fibroids proliferation activity, but also with the inhibition of fibroids apoptosis. This effect may be regulated by progestin.