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80年代以来 ,肝胰脏细小病毒病在养殖中国对虾中广泛流行并造成了一定的损失。作者采用免疫金银染色 (immunogold silver staining,IGSS)技术 ,对患病对虾肝胰脏中的细小病毒(Hepatopancreatic parvovirus,HPV)进行了免疫组织化学的研究。结果表明 ,经 IGSS染色的病虾肝胰脏上皮细胞核内出现了黑色的、圆形或椭圆形、大小为 3～ 15μm的结构。由于该结构与 H- E染色切片上出现的肝胰脏细小病毒包涵体在发生部位、形态和大小上皆一致 ,故而认为该结构为HPV包涵体。证明 ,免疫金银染色法是一种可应用于中国对虾肝胰脏细小病毒病诊断的有效方法。
Since the 1980s, hepatopancreas parvovirus disease has become widespread in farmed shrimp and has caused some losses. The authors used immunogold silver staining (IGSS) to study the immunohistochemistry of Hepatopancreatic parvovirus (HPV) in the hepatopancreas of diseased shrimp. The results showed that black, round or oval, 3 ~ 15μm in size appeared in the nuclei of hepatopancreas epithelial cells of diseased shrimps infected with IGSS. This structure is considered to be an HPV inclusion body because the inclusion body of hepatopancreas and pancreas parvovirus present on the H-E-stained section coincides in the site, the shape and the size of the inclusion body. It is proved that the immunogold-silver staining method is an effective method that can be applied to the diagnosis of hepatopancreas parvovirus in Chinese shrimp.