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利用生理生化方法和电镜观察,对衰老过程中白条纹突变体6001剑叶光合特性进行分析。检测结果表明,该突变体抽穗期和乳熟期的叶绿素含量分别比野生型6028低34.78%和3.00%,而完熟期高于野生型;突变体的净光合速率在抽穗期较低,但在衰老过程中,特别是衰老后期净光合速率的下降显著减缓;Ca2+-ATPase和Mg2+-ATPase酶的活性和光合磷酸化活性也表现出相同的变化趋势;在整个衰老过程中类囊体膜只有68kDa多肽组分发生了明显变化,68kDa多肽组分的含量变化可能是导致光合作用变化的原因之一;电镜观察结果表明,在衰老过程中突变体的叶绿体结构更为完整,叶绿体膜解体缓慢。突变体6001在衰老过程中光合性能衰老相对缓慢,表现出抗衰老特性。
Physiological and biochemical methods and electron microscopy were used to analyze the photosynthetic characteristics of the flag leaf of white striped mutant 6001 during senescence. The results showed that the chlorophyll content of heading stage and milky milky stage were 34.78% and 3.00% lower than that of wild type 6028 respectively, while the maturity stage was higher than that of wild type. The net photosynthetic rate of mutant was lower at heading stage, During the senescence, the decline of net photosynthetic rate significantly slowed down, especially during the late senescence. The activities of Ca2 + -ATPase and Mg2 + -ATPase showed the same trend as photosynthetic phosphorylation activity. The thylakoid membrane had only 68 kDa Polypeptide components changed obviously. The content of 68kDa polypeptide could be one of the reasons that led to the change of photosynthesis. Electron microscopy showed that the chloroplast structure of mutants was more complete and the chloroplast membrane disintegrated slowly during aging. Mutant 6001 photosynthetic properties in aging process is relatively slow aging, showing anti-aging properties.