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研究卵巢癌耐药细胞系OC3/Adr和卵巢癌敏感细胞系OC3在mRNA分子水平上的表达差异。方法 :用高浓度阿霉素间歇诱导法建立卵巢癌耐药细胞系 ;应用mRNA差异显示方法找出两株细胞间cDNA差异表达片段 ;RT PCR检测耐药细胞表达差异片段在卵巢癌细胞和组织中的表达。结果 :建立了稳定传代的卵巢癌耐药细胞系OC3/Adr,耐药指数为15 4;OC3/Adr对VP16,CDDP,5 FU的耐药指数分别11 6,10 5,8 8 ,与亲本细胞相比 ,OC3/Adr细胞形态和周期发生变化。筛选到差异表达片段数十条,对其中的四个片段进行了序列测定和同源性分析 ,S1与精子蛋白高度同源;S2与已定位人18号染色体一段序列高度同源 ,但功能未知 ;S3、S4为未知序列 ,S2在耐药细胞中特异表达 ;S3在卵巢癌与正常卵巢、卵巢良性肿瘤中表达有明显差异。结论 :阿霉素诱导的细胞耐药现象与多基因参与有关 ,细胞对药物的敏感与抵抗本质上受到基因的调控
To study the difference of expression level of OC3 / Adr in ovarian cancer cell line OC3 and ovarian cancer sensitive cell line OC3 at mRNA level. Methods: Intermittent induction with high concentration of doxorubicin was used to establish ovarian cancer resistant cell lines. The cDNA differential expression fragments of two strains were found by mRNA differential display. The expression of differentially expressed fragments in ovarian cancer cells and tissues In the expression. Results: A stable ovarian cancer cell line OC3 / Adr was successfully established and its resistance index was 15 4. The resistance index of OC3 / Adr to VP16, CDDP and 5 FU was 11 6, 10 5 and 8 8, respectively, Compared with cells, OC3 / Adr cell morphology and cycle changes. Several dozens of differentially expressed fragments were screened and four of them were sequenced and analyzed for homology. S1 was highly homologous to sperm protein. S2 was highly homologous to a segment of sequenced human chromosome 18, but its function was unknown ; S3, S4 are unknown sequence, S2 is expressed specifically in drug-resistant cells; S3 is significantly different between ovarian cancer and normal ovary, benign ovarian tumor. CONCLUSION: Adriamycin-induced cell resistance is associated with multiple genes involved. The sensitivity and resistance of cells to drugs are intrinsically regulated by genes