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To explore the anti-inflammatory effect of Cannabis sativa leaf extracts and their repair to damaged skin, a 3D epidermal model (Epikutis?) was used.During the experiment, after epithelial cell surface was pretreated with SLS for 24 h, the anti-inflammatory effect and repair effect of Cannabis sativa leaf extract were observed by testing tissue viability and the representative pro-inflammatory factors such as IL-1α, TNF-α and IL-8. The tissue viability was detected by the MTT test. The results showed that the Cannabis sativa leaf extract effectively inhibited the secretion of inflammatory factors IL-1α, TNF-α and IL-8, and the tissue viability of the skin model was significantly improved after using the sample.The Cannabis sativa leaf extract has a repairing effect on skin barrier damage by inhibiting skin inflammatory reaction.