论文部分内容阅读
目的：观察乌苯美司对白血病细胞生长及淋巴结转移的抑制作用。方法：将ＨＬ－６０，Ｋ５６２，ＭＴ－１，ＭＴ－２，ＭＯＬＴ－４和Ｒａｊｉ白血病细胞分别与乌苯美司培育，计数存活细胞，测定ＭＴ－１和ＭＴ－２的［３Ｈ］ＴｄＲ参入量及Ｐ３８８细胞淋巴结转移。结果：乌苯美司４５００ｍｇ／Ｌ使ＨＬ－６０，ＭＴ－１，ＭＴ－２和Ｒａｊｉ细胞存活数明显减少（Ｐ＜０．０１），０．１，１，１０，１００和１０００ｍｇ／Ｌ可明显抑制ＭＴ－１，ＭＴ－２细胞摄入［３Ｈ］ＴｄＲ（Ｐ＜０．０１）；小鼠每天ｉｐ乌苯美司１，３，１０，３０μｇ／鼠×１１ｄ，转移至淋巴结的Ｐ３８８细胞数明显减少。结论：乌苯美司能抑制人白血病细胞生长及ＭＴ－１，ＭＴ－２细胞的ＤＮＡ合成，有效抑制Ｐ３８８细胞的淋巴结转移。
OBJECTIVE: To observe the inhibitory effect of Ubexime on leukemia cell growth and lymph node metastasis. METHODS: HL-60, K562, MT-1, MT-2, MOLT-4 and Raji leukemia cells were incubated with Ubexime, respectively, and viable cells were counted. The [3H]TdR incorporation of MT-1 and MT-2 was measured. Amount and lymph node metastasis of P388 cells. RESULTS: The survival rate of HL-60, MT-1, MT-2 and Raji cells was significantly decreased (P<0.01) by Ubexime 4500 mg/L, 0.1, 1, 10, 100 and 1000 mg/L. The inhibitory effect of [3H]TdR on MT-1 and MT-2 cells was significantly inhibited (P<0.01); the mice were treated with 1, 3, 10, 30 μg/mouse for 11 days and transferred to lymph node P388 cells. The number is significantly reduced. CONCLUSION: Ubenomes can inhibit the growth of human leukemia cells and the DNA synthesis of MT-1 and MT-2 cells, and effectively inhibit the lymph node metastasis of P388 cells.