采用三种不同方法对泰乐星发酵液进行预处理 ,其中 GSL U絮凝剂效果最好。最适加量为1.0 % ,絮凝 p H7.5。采用离子交换、大网格树脂吸附和有机溶剂萃取三种方法提取泰乐星 ,并进行比较 ,实验得出后两种方法较为合适。吸附法中 ,筛选了 BZ- 84大网格吸附树脂 ,其最适吸附 p H为 8.5～ 9.0 ,用有机溶剂 BT解吸 ,解吸收率达 97%～ 99%。溶剂萃取法中 ,选用乙酸丁酯为萃取剂 ,适宜的萃取 p H为 8.5～ 9.0 ,适当提高萃取温度有利于萃取。 Three different methods were used to pretreat tylosin fermentation broth, of which GSL U flocculant was the best. The optimum dosage is 1.0%, flocculation p H7.5. Three methods of ion exchange, large grid resin adsorption and organic solvent extraction were used to extract tylosin, and the comparison was made. The latter two methods are more suitable. Adsorption method, screening BZ- 84 large grid adsorption resin, the optimum adsorption p H 8.5 ~ 9.0, with organic solvent BT desorption, desorption rate of 97% to 99%. Solvent extraction method, the choice of butyl acetate as extractant, suitable extraction p H 8.5 ~ 9.0, the appropriate increase in extraction temperature is conducive to extraction.