薄层色谱(TLC)是一种分离迅速、效能高、操作简便,可用于分离测定微量物质的分析方法。近年来,由于高效薄层色谱(HPTLC)的应用以及新型高效率的薄层扫描仪的出现,致使薄层定量的精密度和重现性都获得了很大的提高,微量物质用 TLC 直接定量已达到毫微克的水平,越来越受到广大分析工作者的重视。在体内药物分析中,同样也得到了较为广泛的应用,是仅次于HPLC、GC 的一种分析测定方法,适用于药品生物利用度和药物代谢动力学等研究。下面简要介绍近年来薄层定量技术在体内药物分析中的应用概况。一、样品提取测定生物样品中的药物浓度,不仅要求方法灵敏度高,重现性好,而且要求方法具有较高的专属性。通常生物样品在测定前,必须对样品进行净化处理,然后再用适当的方法提取、测定。提取方法通常有下列两种:1.溶剂提取法根据样品中被测物的性质,适当调节样品液的 pH 值,然后选择合 Thin-layer chromatography (TLC) is an analytical method that allows rapid isolation, high efficiency, and ease of use for the separation and determination of trace substances. In recent years, due to the application of High-Performance Thin-Layer Chromatography (HPTLC) and the appearance of new high-efficiency thin-layer scanners, the precision and reproducibility of thin-layer quantification have been greatly improved, and trace substances are directly quantified by TLC. It has reached the nanogram level and is increasingly valued by the majority of analysts. In vivo drug analysis has also been widely used. It is an analytical method that is second only to HPLC and GC. It is suitable for drug bioavailability and pharmacokinetics studies. The following is a brief introduction of the application of thin layer quantification technology in vivo drug analysis in recent years. First, sample extraction Determination of drug concentration in biological samples requires not only high sensitivity and good reproducibility, but also requires high specificity of the method. Before a biological sample is measured, the sample must be purified and then extracted and measured by appropriate methods. Extraction methods usually have the following two: 1. Solvent extraction method According to the nature of the sample in the sample, adjust the pH of the sample solution, then select the appropriate