本文研究了用重组白细胞介素２（ｒＩＬ－２）和含有细胞毒性细胞分化因子（ＣｙｔｏｔｏｘｉｃＣｅｌｌＤｉｆｆｅｒｅｎｔｉａｔｉｏｎＦａｃｔｏｒ，ＣＣＤＦ）的条件培养液，培养正常小鼠脾细胞获得的ＬＩＣＣ在体外的细胞毒作用及其培养条件。结果表明ＬＩＣＣ对体外传代的肿瘤细胞系有一定杀伤活性，对新鲜分离的同基因型肿瘤细胞也有杀瘤效应，对同基因型淋巴母细胞则无杀伤力。细胞的培养活化时间短，需ｒＩＬ－２量较少，诱导产量较高，活性较强。在诱导活化过程中需要Ｍ分泌的ＣＣＤＦ参与，这对于进一步探讨免疫细胞间的相互作用有一定的意义。 In this paper, the in vitro cytotoxicity of cultured mouse spleen cells cultured with recombinant interleukin 2 (rIL-2) and conditioned medium containing cytotoxic cell differentiation factor (CCDF) was studied. . The results showed that LICC had a certain killing activity on tumor cells that were passaged in vitro, and also had tumoricidal effect on freshly isolated isogenic tumor cells, but no killing on isogenic lymphoblasts. The cell culture and activation time is short, the amount of rIL-2 required is small, the induction yield is high, and the activity is strong. In the process of induction activation, CCDF with M secretion is required to participate, which has certain significance for further exploration of the interaction between immune cells.