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目的 探讨基质金属蛋白酶 - 2 (MMP- 2 )、基质金属蛋白酶 - 9(MMP- 9)在肝细胞癌的表达及其与侵袭转移过程中的关系 ,进一步了解肝细胞癌侵袭转移的发生机理。方法 通过免疫组化 SABC法 ,检测 32只大鼠的肝细胞癌模型标本肝癌及癌旁肝组织 MMP- 2、MMP- 9的表达 ,应用图像分析法进行定量分析 MMP- 2、MMP- 9的变化。门静脉癌栓形成作为肝细胞癌侵袭转移标志。结果 免疫组化显示 :癌及其癌旁肝组织均有 MMP- 2、MMP- 9表达 ,MMP - 2、MMP- 9在肝癌组织的表达显著高于癌旁肝组织 (P<0 .0 1) ;MMP- 2、MMP- 9在有侵袭转移肝癌组织的表达显著高于无侵袭转移肝癌组织 (P<0 .0 1)。 MMP- 2、MMP- 9在无侵袭转移肝癌、癌旁肝组织及有侵袭转移癌旁肝组织的表达无显著差异 (P>0 .0 5 )。结论 肝细胞癌在有侵袭转移情况下 MMP- 2、MMP- 9在癌组织表达明显增高。预示可通过检测癌组织 MMP- 2、MMP- 9表达水平帮助判断肝癌复发、转移风险。因此 ,提示其极有可能成为防治肝细胞癌的生物学标志物
Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in hepatocellular carcinoma (HCC) and its relationship with invasion and metastasis, to further understand the mechanism of hepatocellular carcinoma invasion and metastasis. Methods The expression of MMP-2 and MMP-9 in hepatocellular carcinoma and hepatoma tissues from 32 hepatocellular carcinoma models were detected by immunohistochemical SABC method. The quantitative analysis of MMP-2 and MMP-9 was performed by image analysis. Variety. Portal vein tumor thrombosis is a marker of invasion and metastasis of hepatocellular carcinoma. Results Immunohistochemistry showed that the expression of MMP-2 and MMP-9 was found in both cancerous and paraneoplastic liver tissue. The expression of MMP-2 and MMP-9 in hepatocellular carcinoma was significantly higher than that in adjacent liver tissue (P<0.01). The expression of MMP-2 and MMP-9 in invasive metastatic hepatocellular carcinoma was significantly higher than that in non-invasive and metastatic hepatocellular carcinoma (P < 0.01). There was no significant difference in the expression of MMP-2, MMP-9 in liver cancer without invasion and metastasis, para-carcinoma liver tissues, and para-tumor tissues with invasion and metastasis (P>0.05). Conclusions The expression of MMP-2 and MMP-9 in hepatocellular carcinoma was significantly increased in the presence of invasion and metastasis. Predictively, it can help determine the risk of recurrence and metastasis of liver cancer by detecting the expression levels of MMP-2 and MMP-9 in cancer tissues. Therefore, it is highly likely that it will become a biological marker for the prevention and treatment of hepatocellular carcinoma.