论文部分内容阅读
研究了槲蕨(Drynaria roosii)孢子的无菌培养和常规繁殖方式,观察记录消毒方式、无机盐浓度和光照强度对槲蕨孢子萌发和配子体发育的影响。结果表明:槲蕨孢子接种后6～7d即能萌发,60d左右发育形成心形原叶体,100d左右开始形成孢子体幼苗;采用NaClO消毒5.5min左右是理想的消毒方式;在Knops培养基中孢子的萌发率最高,可达49%左右。低无机盐浓度的MS培养基有利于孢子萌发,但不利于配子体发育;黑暗条件下孢子能萌发但不能形成片状体。采用5%NaClO表面灭菌5.5min,1/2MS培养基在40μmol.m-2.s-1光照条件下孢子萌发和配子体发育良好。
The sterile culture and conventional breeding methods of Drynaria roosii spores were studied. The effects of sterilization mode, inorganic salt concentration and light intensity on spore germination and gametophyte development were observed. The results showed that the spores could germinate 6 ~ 7 days after inoculation, and the spheroplasts began to form after about 60 days. Sporophyte seedlings began to form around 100 days. NaClO disinfection for 5.5 minutes was the ideal disinfection method. Spores The highest germination rate, up to about 49%. MS medium with low inorganic salt concentration is good for spore germination, but not conducive to the development of gametophytes; spores can germinate but can not form sheet-like body under dark conditions. The surface was sterilized with 5% NaClO for 5.5 min, and spore germination and gametophytes developed well in 1 / 2MS medium under 40μmol.m-2.s-1 light.