论文部分内容阅读
目的研究猪苓多糖(Polyporus umbellatus polysaccharides,PPS)活化小鼠骨髓来源树突状细胞(bone marrow dendritic cells,BMDC)功能调节的作用机制,进一步阐明PPS的免疫学活性机制。方法以3H-TdR掺入法、ELISA及流式细胞术检测BMDC表型和功能的各项指标。结果 PPS刺激小鼠BMDC表达CD11c、CD86及白细胞介素-12、-10(IL-12、IL-10)产生,并且具有剂量依赖效应。另外,较阴性对照组,经PPS诱导成熟的BMDC其活化初始T细胞的能力显著提高而吞噬能力显著下降。抗小鼠Toll样受体4(TLR4)单抗可抑制PPS刺激BMDC产生IL-12 p40及阻断荧光标记猪苓多糖(fluorescence-labeled PPS,f-PPS)与BMDC的结合,而抗TLR2及补体受体3(CR3)单抗却无此效应。结论 PPS可经TLR4活化小鼠BMDC发挥免疫调节活性。
Objective To study the mechanism of functional regulation of bone marrow dendritic cells (BMDC) activated by Polyporus umbellatus polysaccharides (PPS) in mice and further elucidate the immunological mechanism of PPS. Methods 3H-TdR incorporation, ELISA and flow cytometry were used to detect the phenotype and function of BMDC. Results PPS stimulated the expression of CD11c, CD86 and interleukin-12, IL-10 and IL-10 in BMDC of mice and had a dose-dependent effect. In addition, compared with the negative control group, PDC-induced maturation BMDC significantly increased the ability to activate naive T cells and phagocytosis ability was significantly decreased. Anti-mouse Toll-like receptor 4 (TLR4) monoclonal antibody inhibited the binding of BMDC to IL-12 p40 and fluorescence-labeled P-CSF to BMDC by PPS, while the anti-TLR2 and Complement Receptor 3 (CR3) mAb did not. Conclusion PPS can activate immunomodulatory activity in mouse BMDC via TLR4 activation.