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目的观察地理位置对中华白蛉基因多态性及形态学的影响。方法选择3地采集的5组中华白蛉,用扫描电镜观察了它们的咽甲,并用5种单一的10bp引物对其基因组DNA随机扩增多态性进行了比较。结果在扫描电镜下,5组中华白蛉的咽甲形态基本一致,各组间未见明显不同。3叶咽甲的形态也无大的区别。5组白蛉的基因组DNA共扩增出138个条带,有些条带为5组白蛉所共有,大多为组间共有,个别为个体特异性条带。这样,受检的3地5组白蛉就可根据其由数种单一10bp引物扩增的基因多态性图谱区分开来。聚类分析结果表明:四川南坪的中华白蛉其亲缘关系介于山西吉县和陕西宜川的白蛉之间。结论3地采集的5组中华白蛉虽然在形态上有某些差别,但仍属同一生物种。
Objective To observe the effect of geographical location on the polymorphism and morphology of Chinese white foxtail Methods Five Chinese white shoots collected from three sites were selected and their pharyngeal tissues were observed with scanning electron microscopy. Five single 10 bp primers were used to compare the genomic DNA random amplified polymorphisms. Results Under the scanning electron microscope, the morphology of pharyngeal pharynx in the five groups was basically the same, and no significant difference was found among the groups. 3 leaf pharynx A form of no major difference. A total of 138 bands were amplified from the genomic DNA of the five groups of white sandwiches. Some of the bands were shared by the five groups of white sandwiches, mostly shared by groups and individual bands were individual. In this way, three groups of five batches of subjects tested were distinguished by their genetic polymorphisms amplified by several single 10 bp primers. The results of cluster analysis showed that the genetic relationship between Chinese white pomfret in Nanping of Sichuan was between that of Jixian in Shanxi Province and that of Yichuan in Shaanxi Province. Conclusions Although there are some differences in the morphological characteristics of the 5 groups of white catfish collected from 3 sites, they still belong to the same species.