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髓鞘形成是一个包括协同性的胞吐、内吞、mRNA转运和细胞骨架的动态变化的复杂过程。尽管髓鞘的异常在溶酶体贮积症中很常见,但对溶酶体在髓鞘形成和维持中所扮演的角色仍不清楚。本文发现Schwann细胞中的晚期内涵体/溶酶体包含大量的髓鞘蛋白P0,含量占超过一半的外周神经系统中的致密髓鞘的总蛋白并且在不同的刺激下表现出Ca2+依赖性的胞吐作用。Rab27a(一种将分泌溶酶体运输至细胞膜的小GTP酶)下调,极大地阻碍了Schwann细胞中的溶酶体胞吐作用,减少了再生坐骨神经的髓鞘形成。这些发现强调了Schwann细胞中溶酶体的新角色,提示调节Schwann细胞中的溶酶体胞吐作用在外周神经系统中有很重要的生理和病理意义。
Myelination is a complex process that involves synergistic exocytosis, endocytosis, mRNA translocation, and dynamic changes in the cytoskeleton. Although myelin abnormalities are common in lysosomal storage disease, the role of lysosomes in myelination and maintenance remains unclear. It has been found herein that late endosomes / lysosomes in Schwann cells contain a large amount of myelin P0, which accounts for more than half of the total myelin total protein in the peripheral nervous system and shows Ca2 + -dependent cells under different stimuli Spit effect. Down-regulation of Rab27a, a small GTPase secreting lysosomes to the cell membrane, greatly hinders lysosome exocytosis in Schwann cells and reduces myelination of regenerative sciatic nerves. These findings underscore the new role of lysosomes in Schwann cells, suggesting that regulation of lysosome exocytosis in Schwann cells has important physiological and pathological implications in the peripheral nervous system.