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以体外培养的人胚肺二倍体成纤维细胞为材料,当细胞培养至27代时,将细胞分为加黄芪提取液的给药组和不加黄芪的对照组,相同条件下传代培养,直至细胞停止增殖。同时,每隔一定代龄用流式细胞计测定两组细胞的增殖能力,以化学发光法测定其 SOD 含量,并进行常规染色体检查和细胞一代存活时间的实验。结果表明:给药组生长77代,而对照组细胞仅存活至52代。至传代终止两组细胞都始终保持其二倍体性质,随着代龄的增加,两组细胞的增殖能力、SOD 含量和一代存活时间均明显下降。同一代龄,给药组细胞的增殖能力、SOD 含量和一代存活时间高于对照组,随代龄的增加,这种差距更加明显。
For human embryonic lung diploid fibroblasts cultured in vitro, when the cells were cultured to the 27th generation, the cells were divided into a treatment group with astragalus extract and a control group without astragalus, and subcultured under the same conditions. Until the cell stops growing. At the same time, the proliferative capacity of the two groups of cells was determined by a flow cytometer at a certain age, and the SOD content was measured by a chemiluminescence method, and routine chromosomal examinations and cell survival test were performed. The results showed that the administration group grew for 77 generations while the control group cells survived only to 52 generations. The two groups of cells maintained their diploid properties until the end of passage, and as the age of the two groups increased, the proliferation ability, SOD content, and one-generation survival time of the two groups of cells were significantly decreased. At the same age, the proliferative capacity, SOD content, and one-year survival time of the cells in the administration group were higher than those in the control group, and this difference was more pronounced with the increase of the age of generation.