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研究地塞米松、ｒＩＬ－１ｒａ对内皮细胞表达粘附分子的影响，为炎症反应的病理过程提供理论依据。用ｒＴＮＦα、ｒＩＬ－１和ＬＰＳ诱导培养的人脐静脉内皮细胞（ＨＵＶＥＣ），以地塞米松或ｒＩＬ－１ｒａ处理ＬＰＳ或ｒＩＬ－１诱导的ＨＵＶＥＣ，采用Ｃｅｌ－ＥＬＩＳＡ法检测粘附分子ＩＣＭＡ－１和ＥＬＡＭ－１的表达。结果：ｒＴＮＦα、ｒＩＬ－１和ＬＰＳ提高ＨＵＶＥＣ表达ＩＣＡＭ－１和ＥＬＡＭ－１；地塞米松、ｒＩＬ－１ｒａ抑制ｒＩＬ－１诱导的粘附分子表达。表明地塞米松、ｒＩＬ－１ｒａ以不同机制抑制ＨＵＶＥＣ表达ＩＣＡＭ－１和ＥＬＡＭ－１。
To study the effect of dexamethasone and rIL-1ra on the expression of adhesion molecules in endothelial cells and to provide a theoretical basis for the pathological process of inflammatory response. Cultured human umbilical vein endothelial cells (HUVECs) were induced with rTNFα, rIL-1 and LPS, HUVECs induced by LPS or rIL-1 were treated with dexamethasone or rIL-1ra, and the adhesion molecules ICMA-1 And ELAM-1 expression. Results: rTNFα, rIL-1 and LPS enhanced the expression of ICAM-1 and ELAM-1 in HUVECs. Dexamethasone and rIL-1ra inhibited the expression of rIL-1-induced adhesion molecules. Dexamethasone showed that rIL-1ra inhibited the expression of ICAM-1 and ELAM-1 in HUVEC by different mechanisms.