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目的考察柴胡皂苷a、d(中药柴胡有效成分,为一对差向异构体,结构相近)在柴胡煎煮过程中的溶出和转化行为。方法采用HPLC测定柴胡煎煮过程中煎液和药渣在不同时间点各柴胡皂苷的含量变化。色谱条件为WondaSil C18色谱柱(4.6 mm×250 mm,5μm);流动相:甲醇-水(53∶47);流速:1.0 mL·min-1;检测波长:210,254 nm。结果柴胡皂苷a、d在柴胡水煎剂中的溶出和转化行为差异较大。主要体现在:1柴胡水煎液中可以检测到柴胡皂苷a,但检测不到柴胡皂苷d,药渣中柴胡皂苷a和d均能检测到;2无论在煎液还是在药渣中,柴胡皂苷a均少部分降解为柴胡皂苷b1,而柴胡皂苷d在水煎剂中全部转化为柴胡皂苷b2,在药渣中部分转化为柴胡皂苷b2。结论柴胡水煎液中不存在柴胡皂苷d,取而代之的是其转化产物柴胡皂苷b2。本实验为从水煎剂的角度认识柴胡的有效成分提供参考。
Objective To investigate the dissolution and transformation behavior of saikosaponin a, d (the effective components of Bupleurum chinense, a pair of epimers, similar in structure) during the decoction of Radix Bupleuri. Methods The contents of saikosaponins in decoction and dregs of Chaihu decoction were determined by HPLC at different time points. The chromatographic conditions were WondaSil C18 column (4.6 mm × 250 mm, 5 μm); mobile phase: methanol-water (53:47); flow rate: 1.0 mL · min-1; detection wavelength: 210,254 nm. Results Saikosaponin a, d in the Bupleurum decoction dissolution and conversion behavior are quite different. Mainly reflected in: 1 Bupleurum decoction can detect saikosaponin a, but not detected saikosaponin d, dregs of saikosaponin a and d can be detected; 2 both in decoction or medicine Residue, saikosaponin a are a small part of the degradation of saikosaponin b1, and saikosaponin d in the decoction all converted to saikosaponin b2, part of the dregs into saikosaponin b2. Conclusion Bupleurum saponin d does not exist in the decoction of Bupleurum, instead, its transformation product saikosaponin b2. The experiment for the decoction of Bupleurum understanding of the active ingredients provide a reference.