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背景:骨肿瘤动物模型是骨肿瘤基础研究的重要工具,但现有模型的局限性导致其无法满足研究需要。目的目的:构建兔髂骨种植瘤模型,并观察其基本特性。方法方法:30只健康新西兰大白兔,分为实验组26只、对照组4只。外科无菌条件下,切取荷瘤兔VX2肿瘤,切成小块,种植于健康大白兔的双侧髂骨,分别于术前、术后1~6周观察动物的全身情况、体重及行为改变,行骨盆及肺部X线片及CT平扫检查,同时行髂骨手术部位、肺、肝、肾等脏器组织的病理检查。结果结果:26只52侧肿瘤种植部位,50侧成瘤,成瘤率为96.1%。动物体重从术后第3周开始下降,除术后1~6周随机处理的18只动物,另8只自然死亡动物的术后存活期为30~49 d,平均40.3 d。X线片检查从第3周开始,CT平扫检查从第2周开始,均可见髂骨溶骨性破坏表现;CT平扫检查从第3周可见明显软组织包块,第5周可见明显的肺部肿瘤转移征象。病理检查发现肿瘤从第2周开始侵及骨外肌肉,第3周出现肺转移。结论结论:兔髂骨种植瘤模型具有成瘤率高、操作简单、影像学特征典型、与人类骨肿瘤相似度较高等优点,可用于骨肿瘤外科治疗、影像学检查等基础研究。
BACKGROUND: Animal models of bone tumors are important tools for the basic research of bone tumors. However, the limitations of the existing models make them unable to meet the research needs. Objective: To construct a model of ilium ilium implantation tumor and observe its basic characteristics. Methods and Methods: Thirty healthy New Zealand rabbits were divided into experimental group (n = 26) and control group (n = 4). Under surgical aseptic conditions, tumor-bearing rabbit VX2 tumors were excised and cut into small pieces and planted in the bilateral iliac bone of healthy rabbits. Whole body conditions, body weight and behavioral changes were observed before and 1 to 6 weeks after operation , Pelvis and lung X-ray and CT plain examination, at the same time iliac surgery site, lung, liver, kidney and other organs of the pathological examination. Results: Twenty-six 52-sided tumor sites and 50 tumors formed a tumor formation rate of 96.1%. The body weight of animals decreased from the third week after operation, except for 18 animals randomly treated from 1 to 6 weeks after operation. The survival time of the other 8 naturally dead animals ranged from 30 to 49 days (average 40.3 days). X-ray examination from the beginning of the third week, CT scan from the first 2 weeks, all showed osteolytic destruction of iliac bone; CT scan showed obvious soft tissue mass from the third week, the first 5 weeks showed obvious Lung tumor metastasis signs. Pathological examination revealed that the tumor invaded the extra-muscular muscles from the second week and lung metastases occurred in the third week. Conclusion: The ilium implanted tumor model has the advantages of high tumor-forming rate, simple operation, typical imaging features and high similarity with human bone tumors. It can be used in the basic research of surgical treatment and imaging examination of bone tumors.