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1 背景蛋白质组分析的三大主要步骤是 :(1)分离复杂的蛋白质混合物 ;(2 )鉴定分离出的多肽 ;(3)数据库比对。与此相对应的三大核心技术分别是二维凝胶电泳技术、质谱技术、生物信息学。对于分离复杂的蛋白质混合物 ,二维凝胶电泳是目前惟一能同时分辨上千乃至上万种蛋白质的技
1 Background Three major steps in proteomic analysis are: (1) separation of complex protein mixtures; (2) identification of isolated polypeptides; (3) database alignment. Corresponding to the three core technologies are two-dimensional gel electrophoresis, mass spectrometry, bioinformatics. For the separation of complex protein mixtures, two-dimensional gel electrophoresis is currently the only technology that can distinguish between thousands and even tens of thousands of proteins simultaneously